Journal: International Journal of Molecular and Cellular Medicine

Article Title: Potential Protective Effects of Cerium Oxide Nanoparticles on Ultraviolet-B Irradiated Fibroblast Cells

doi: 10.22088/IJMCM.BUMS.14.3.828

Figure Lengend Snippet: CNP concentrations of 10, 50, and 100 μM display non-toxicity for L929 fibroblasts with significant increases in cell viability compared to the UVB irradiated group. (A) L929 fibroblasts were treated with different concentrations (10, 50, and 100 μM) of CNP. (B) L929 fibroblasts were irradiated at different intensities of UVB radiation (150-900 mJ/cm2). (C) L929 fibroblasts were treated with 10, 50, and 100 μM CNP for 24 hours before irradiation with 600 mJ/cm2 UVB. (Control) cells non-treated and non-irradiated, (UVB) cells irradiated with 600 mJ/cm2 UVB and non-treated, (10 μM + UVB) cells treated with 10 μM of CNP and irradiated, (50 μM + UVB) cells treated with 50 μM of CNP and irradiated, (100 μM + UVB) cells treated with 100 μM of CNP and irradiated. The results are expressed as a percentage of control. Each column represents the mean ± SD (n = 3). (B): **p = 0.0017, ****p < 0.0001 significant difference compared with ontrol. (C): ####p < 0.0001 significant difference compared with Control. ****p < 0.0001 significant difference compared with UVB.

Article Snippet: L929 fibroblast cells (subcutaneous connective mouse tissue), purchased from Pasteur Institute (Tehran, Iran), were cultured in RPMI 1640, 10% FBS, and 1% penicillin-streptomycin in humidified surroundings with 5% carbon dioxide (CO2) under 37 ° C temperature conditions.

Techniques: Irradiation, Control

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Potential Protective Effects of Cerium Oxide Nanoparticles on Ultraviolet-B Irradiated Fibroblast Cells

doi: 10.22088/IJMCM.BUMS.14.3.828

Figure Lengend Snippet: CNP leads to an increase in total antioxidant capacity in L929 fibroblasts compared to the control group. (Control) cells non-treated and non-irradiated, (CNP) cells treated with 50 μM of CNP. (A) Standard curve was plotted at Trolox concentrations of 0, 40, 80, 160, 200, and 400 nmol/mL at the absorption wavelength of 450 nm, (B) CNP leads to a significant increase in total antioxidant capacity in L929 fibroblasts compared to the control group. According to the standard curve line equation of Trolox (y = 0.0009x + 0.0188, R2 = 0.985) the antioxidant concentration of the control group is 34.66 ± 1.57 nmol/mL and the treated group with a concentration of 50 μM is 118 ± 4.716 nmol/mL. **p = 0.0018 significant difference compared with Control.

Article Snippet: L929 fibroblast cells (subcutaneous connective mouse tissue), purchased from Pasteur Institute (Tehran, Iran), were cultured in RPMI 1640, 10% FBS, and 1% penicillin-streptomycin in humidified surroundings with 5% carbon dioxide (CO2) under 37 ° C temperature conditions.

Techniques: Control, Irradiation, Concentration Assay

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Potential Protective Effects of Cerium Oxide Nanoparticles on Ultraviolet-B Irradiated Fibroblast Cells

doi: 10.22088/IJMCM.BUMS.14.3.828

Figure Lengend Snippet: Figure 4. Reduction in ROS produced in L929 cells pretreated with 50 μM CNP concentrations and irradiated with UVB in comparison to the UVB irradiated group. Intracellular ROS were measured by flow cytometry using an oxidation-sensitive fluorescent probe, DCFH-DA which is oxidized to DCF in the presence of ROS. (Control) cells non-treated and non-irradiated, (UVB) cells irradiated with 600 mJ/cm2 UVB and non-treated, (CNP + UVB) cells treated for 24 hours with 50 μM CNP and irradiated, (CNP) cells treated for 24 hours with 50 μM CNP. (A) The intracellular ROS histogram is presented for all groups. FL1-H+ indicates positive intracellular ROS. FL1-H- indicates negative intracellular ROS. Guidelines have been drawn through histograms for means of comparison. (B) Quantification of the fluorescence intensities of the images in panel (A). The geometric mean indicates the intensity of fluorescence emitted from cells containing ROS, which is a more accurate criterion for reporting. Each column represents the mean ± SD (n = 3). ###p = 0.0003 significant difference compared with Control. **p = 0.003 significant difference compared with UVB.

Article Snippet: L929 fibroblast cells (subcutaneous connective mouse tissue), purchased from Pasteur Institute (Tehran, Iran), were cultured in RPMI 1640, 10% FBS, and 1% penicillin-streptomycin in humidified surroundings with 5% carbon dioxide (CO2) under 37 ° C temperature conditions.

Techniques: Produced, Irradiation, Comparison, Flow Cytometry, Control, Fluorescence

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Potential Protective Effects of Cerium Oxide Nanoparticles on Ultraviolet-B Irradiated Fibroblast Cells

doi: 10.22088/IJMCM.BUMS.14.3.828

Figure Lengend Snippet: Reduction in generated apoptosis in L929 cells pretreated with 50 μM CNP concentrations and irradiated with UVB in comparison to the UVB irradiated group . Percentage of the apoptotic L929 cells pretreated with CNP and irradiated with UVB measured by flow cytometry using Annexin V‐FITC and PI double staining. (Control) cells non-treated and non-irradiated, (UVB) cells irradiated with 600 mJ/cm 2 UVB and non-treated, (CNP + UVB) cells treated for 24 hours with 50 μM CNP and irradiated, (CNP) cells treated for 24 hours with 50 μM CNP. (A) Representative dot plots of annexin V/PI staining of L929 cells (bottom left quadrant (Q4): viable cells, bottom right quadrant (Q3): cells undergoing apoptosis, top right quadrant (Q2): dead cells in the late stage of apoptosis, top left quadrant (Q1): Necrotic cells). (B) General Apoptosis, ##p = 0 .0018 significant difference compared with Control . **p = 0.0079 significant difference compared with UVB. (C) Necrotic cells, **p = 0.0099 significant difference compared with Control. #p = 0.0332 significant difference compared with CNP. *p = 0.0245 significant difference compared with CNP. (D) Late apoptotic cells, ## # p = 0.0007 significant difference compared with Control. **p = 0.0043 significant difference compared with UVB. (E) Early apoptotic cells. (F) Live cells, #p = 0.0238 significant difference compared with Control, ##p = 0.0062 significant difference compared with Control, *p = 0.0339 significant difference compared with UVB. Each column represents the mean ± SD (n = 3).

Article Snippet: L929 fibroblast cells (subcutaneous connective mouse tissue), purchased from Pasteur Institute (Tehran, Iran), were cultured in RPMI 1640, 10% FBS, and 1% penicillin-streptomycin in humidified surroundings with 5% carbon dioxide (CO2) under 37 ° C temperature conditions.

Techniques: Generated, Irradiation, Comparison, Flow Cytometry, Double Staining, Control, Staining

Journal: International Journal of Biomaterials

Article Title: Blends of Thermoplastic Polyurethane and Polydimethylsiloxane Rubber: Assessment of Biocompatibility and Suture Holding Strength of Membranes

doi: 10.1155/2013/240631

Figure Lengend Snippet: L929 cells incubated with (a) positive control, (b) negative control, and (c) 50 : 50 blend of TPU : PDMS containing 5 pbw of compatibilizer over 24 h.

Article Snippet: The in vitro cytotoxicity was assessed as per ISO-10993-5 (2002) using L929 mouse fibroblast subcutaneous connective tissue cell line procured from National Centre for Cell Sciences, Pune, India.

Techniques: Incubation, Positive Control, Negative Control

Journal: International Journal of Biomaterials

Article Title: Blends of Thermoplastic Polyurethane and Polydimethylsiloxane Rubber: Assessment of Biocompatibility and Suture Holding Strength of Membranes

doi: 10.1155/2013/240631

Figure Lengend Snippet: L929 cells incubated with (a) positive control, (b) negative control, and (c) 50 : 50 blend of TPU : PDMS containing 5 pbw of compatibilizer over 24 h.

Article Snippet: The in vitro cytotoxicity was assessed as per ISO-10993-5 (2002) using L929 mouse fibroblast subcutaneous connective tissue cell line procured from National Centre for Cell Sciences, Pune, India.

Techniques: Incubation, Positive Control, Negative Control